Figure 3.
Effect of monoclonal antibodies to HKa light chain and heavy chain on release of IL-1β by HKa, GST-D5 and GST-D3. Mononuclear cells (2.0×106/mL) were incubated with 1.2 μmol/L of C11C1, or 2B5 for 30 minutes at 37°C. HKa, GST-D5, GST-D3, or IgG were then added and incubated for 60 minutes at 37°C and processed as in Figure 1. Dotted line indicates IL-1β concentration at 0 minutes with monocytes alone.