Effect of monoclonal antibodies to uPAR, Mac-1, LFA-1 ((CD11a/CD18) and gC1qR on release of IL-1β by HKa, GST-D5, GST-D3, and GST-E7P. Mononuclear cells (2.0×106/mL) were incubated with antibodies to uPAR (31 nM), Mac-1 (62 nM), LFA-1 (31 nM), and gC1qR (10, 30,100 nM) for 10 minutes at 37°C. HKa, GST-D5, GST-D3, or GST-E7P (600 nM) were then added and incubated for 60 minutes at 37°C and processed as in Figure 1.