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eNDK has 3′ repair phosphodiesterase activity. (A) Schematic view of oligonucleotide substrate used in this experiment. Asterisk indicates the radioactively labeled terminus. (B) Time course of phosphoglycolate removal from the 3′ end of the oligonucleotide by eNDK and EndoIV (0-20 min) and ExoIII (0-5 min). Enzymes and substrates used are indicated above lanes. Products are displayed on a 20% urea/PAGE gel. See text for further details.
