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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Transplant Proc. 2008 Mar;40(2):638–640. doi: 10.1016/j.transproceed.2008.02.013

Table 2.

Inhibition of SREBP with 25-OH Cholesterol Blocks the Induction of Protection From Complement by IL-4 in Porcine Endothelial Cells

% Cytotoxicity
Medium 70.5 ± 1.7
IL-4 12.7 ± 4.2
25 OH-Cholesterol 75.4 ± 1.3
25 OH-Cholesterol + IL-4 72.6 ± 1.9

Primary cultures of porcine aortic ECs were treated with either medium, 10 ng/mL IL-4, 10 µmol/L 25-OH cholesterol alone or in combination with 10 ng/mL rIL-4 for 48 hours at 37°C. ECs were then incubated with 15% human serum for 2 hours and killing assessed by a neutral red vital dye uptake assay.