Table 2.
% Cytotoxicity | |
---|---|
Medium | 70.5 ± 1.7 |
IL-4 | 12.7 ± 4.2 |
25 OH-Cholesterol | 75.4 ± 1.3 |
25 OH-Cholesterol + IL-4 | 72.6 ± 1.9 |
Primary cultures of porcine aortic ECs were treated with either medium, 10 ng/mL IL-4, 10 µmol/L 25-OH cholesterol alone or in combination with 10 ng/mL rIL-4 for 48 hours at 37°C. ECs were then incubated with 15% human serum for 2 hours and killing assessed by a neutral red vital dye uptake assay.