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. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Microsc Res Tech. 2008 Dec;71(12):887–896. doi: 10.1002/jemt.20634

Fig. 1.

Fig. 1

An experimental design for measuring ultrafast optical pulse characteristics through optical fibers and the resultant two-photon excitation fluorescence microscopy performance. The experimental apparatus consists of a pulse compressor, an optical fiber coupling system including a fiber focuser and a fiber collimator, a two-photon excitation laser scanning fluorescence microscope. The pulse compressor provides linear prechirping to compensate for chromatic dispersion in the system. The optical pulse profile can be measured with an optical autocorrelator and a spectrum analyzer. The delivered optical pulses enter into a two-photon excitation fluorescence microscope where the TPE and the optical resolution are measured.