Figure 3.
Methylation of the Oct4 promoter and the H19 DMR in colon epithelial cells was not affected by folate-deficient diet. Methylation status of the Oct4 promoter (A) and the H19 DMR (B) in colon epithelial cells analyzed using bisulfite sequencing, covering 12 and 15 CpG sites, respectively. Samples derived from control diet (control, n = 5) or folate-deficient diet (n = 5); in each case, 3 samples were taken from wild-type (WT) mice and 2 samples from Ung−/− mice. Black boxesindicate methylated CpGs, white boxes unmethylated CpGs. KO, knockout.