Table 3.
λ CII Mutant Analysis
| Ung−/− | Ung−/− | |||
|---|---|---|---|---|
| WT control | WT deficient | Control | deficient | |
| No. of mice | 8 | 10 | 8 | 9 |
| Mutants sequenced | 108 | 194 | 215 | 213 |
| Sequences with CII mutation | 82 | 158 | 154 | 161 |
| Nonredundant eventsa | 70 | 120 | 133 | 119 |
| Transition | 46 (66%) | 63 (53%) | 89 (67%) | 70 (59%) |
| C/G → T/A | 40 (57%) | 49 (41%) | 67 (50%) | 66 (55%) |
| non CpG | 2 (3%) | 8 (7%) | 21 (16%) | 20 (17%) |
| at CpG | 38 (54%) | 41 (34%) | 46 (35%) | 46 (39%) |
| T/A → C/G | 6 (9%) | 14 (12%) | 22 (17%) | 4 (3%) |
| Transversion | 20 (29%) | 43 (36%) | 29 (22%) | 33 (28%) |
| C/G → A/T | 12 (17%) | 24 (20%) | 22 (17%) | 28 (24%) |
| C/G → G/C | 4 (6%) | 6 (5%) | 2 (2%) | 2 (2%) |
| T/A → A/T | 3 (4%) | 8 (7%) | 3 (2%) | 3 (3%) |
| T/A → G/C | 1 (1%) | 5 (4%) | 2 (2%) | 0 |
| Events at T/A | 10 (14%) | 27 (23%) | 27 (20%) | 7 (6%) |
| Frameshift | 4 (6%) | 14 (12%) | 15 (11%) | 16 (13%) |
| +1 | 0 | 1 (1%) | 1 (1%) | 0 |
| − 1 | 4 (6%) | 13 (11%) | 14 (11%) | 16 (13%) |
WT, wild-type.
a
Identical mutations occurring at the same site in more than one phage from the same mouse (jackpot mutants) were tabulated as a single mutational event. The difference between C:G transition frequency at non–CpG sites of Ung−/− mice and WT mice is significant (P = .0003, Fisher’s exact test). For sequencing and analysis of mutation patterns, all available mutant plaques were used.