Table 3.
Western blot conditions and list of antibodies used in the present study
| Antigen | Source | Host Animal | Blocking Buffer | Primary Antibody Dilution | Secondary Antibody Dilution |
|---|---|---|---|---|---|
| ACE2 | Santa Cruz Biotechnology (catalog no. sc-17719) | Goat | 7% milk | 1:300 | 1:1,500 |
| Nox1 | Santa Cruz Biotechnology (catalog no. sc-5821) | Goat | 7% milk | 1:300 | 1:1,500 |
| Nox4 | Santa Cruz Biotechnology (catalog no. sc-30141) | Rabbit | 7% milk | 1:500 | 1:1,000 |
| PKC-βI | Santa Cruz Biotechnology (catalog no. sc-209) | Rabbit | 7% milk | 1:1,000 | 1:1,000 |
| Phospho-PKC-βII | Upstate (catalog no. 07-873) | Rabbit | 5% BSA | 1:1,000 | 1:1,000 |
| PKC-βII | Santa Cruz Biotechnology (catalog no. sc-210) | Rabbit | 7% milk | 1:300 | 1:1,000 |
| Phospho-EGFR | Cell Signaling Technology (catalog no. 2236) | Rabbit | 3% BSA | 1:1,000 | 1:1,000 |
| EGFR | Santa Cruz Biotechnology (catalog no. sc-03) | Rabbit | 5% milk | 1:1,000 | 1:1,000 |
| VSMC α-actin | Sigma-Aldrich (catalog no. A5228) | Mouse | 1% casein (Bio-Rad) | 1:20,000 | 1:1,000 |
| Anti-rabbit antibody | Santa Cruz Biotechnology (catalog no. sc-2313) | Donkey | 7% milk | ||
| Anti-goat antibody | Santa Cruz Biotechnology (catalog no. sc-2020) | Donkey | 7% milk | ||
| Anti-mouse antibody | GE Healthcare (catalog no. NXA 931) | Sheep | 7% milk |
EGFR, EGF receptor; VSMC, vascular smooth muscle cell.