Table 2.
Sample means, within-visit standard deviations, reliability coefficients (R), and CVs for replicate samples drawn from a single venipuncture (substudy 2), ARIC Carotid MRI Study 2005-2006
| Mean on QC pairs |
Within-visit SD |
R |
CV |
|||||
|---|---|---|---|---|---|---|---|---|
| % gated | MFI | % gated | MFI | % gated | MFI | % gated | MFI | |
| Leukocyte markers | ||||||||
| Monocytes | ||||||||
| CD14+ | 100.0 | 113.1 | 0.0 | 4.1 | 0.35 | 0.97 | 0.0 | 3.7 |
| CD14+/TLR-2+ | 66.0 | 14.2 | 2.9 | 0.3 | 0.93 | 0.92 | 4.4 | 1.9 |
| CD14+/TLR-4+ | 64.6 | 16.1 | 1.3 | 0.4 | 0.89 | 0.85 | 2.1 | 2.6 |
| CD14+/CD41+/TLR-4+ | 10.9 | 47.4 | 1.0 | 3.3 | 0.80 | 0.63 | 9.5 | 6.9 |
| CD14+/CD162+ | 100.0 | 116.1 | 0.0 | 3.8 | 0.35 | 0.94 | 0.0 | 3.3 |
| CD14+/TLR-2+/CD162+ | 67.6 | 14.0 | 2.9 | 0.3 | 0.92 | 0.92 | 4.3 | 2.0 |
| CD14+/COX-2+ | 98.0 | 16.7 | 1.3 | 1.3 | 0.69 | 0.73 | 1.3 | 7.8 |
| CD45+ | 94.4 | 77.9 | 1.1 | 2.9 | 0.87 | 0.91 | 1.1 | 3.8 |
| CD45+/MPO+ | 94.4 | 92.8 | 1.1 | 7.7 | 0.87 | 0.90 | 1.1 | 8.3 |
| Granulocytes | ||||||||
| CD162+ | 99.9 | 81.2 | 0.1 | 2.6 | 0.67 | 0.97 | 0.1 | 3.2 |
| MPO+ | — | 845.2 | — | 32.7 | — | 0.96 | — | 3.9 |
| Lymphocytes | ||||||||
| CD162+ | 88.8 | 55.1 | 1.5 | 1.7 | 0.97 | 0.97 | 1.7 | 3.1 |
| Platelet markers | ||||||||
| Total platelets CD61+ | 5.5 | 64.7 | 0.5 | 1.9 | 0.91 | 0.99 | 8.8 | 3.0 |
| Single platelets CD61+ | 85.5 | — | 1.5 | — | 0.81 | — | 1.8 | — |
| CD61+/CD62P+ | 29.8 | 21.8 | 6.0 | 1.5 | 0.82 | 0.87 | 20.2 | 6.7 |
| CD41+ | 95.1 | 80.8 | 1.1 | 3.5 | 0.88 | 0.91 | 1.1 | 4.3 |
| CD41+/CD154+ | 3.4 | 12.0 | 0.9 | 0.4 | 0.80 | 0.71 | 25.6 | 3.6 |
| Cell aggregates | ||||||||
| Platelet-monocyte | 17.4 | 48.4 | 1.2 | 2.9 | 0.89 | 0.76 | 7.1 | 6.0 |
| Platelet-granulocyte | 16.8 | 50.3 | 1.2 | 1.8 | 0.89 | 0.91 | 7.0 | 3.5 |
| Platelet-lymphocyte | 16.8 | 49.2 | 1.1 | 2.6 | 0.91 | 0.85 | 6.6 | 5.3 |
n = 112 replicate pairs. Total platelets include single (activated and nonactivated) platelets and platelet-platelet aggregates. —, measures not available.