Figure 3.

Effect of pantethine (P) and glutathione (G) on chaperone-like activity of α-crystallin on the aggregation of ADH at 37°C. (A) Protein concentrations of ADH and α-crystallin were adjusted to approximately 2:1 (ADH/α) to provide partial protection against aggregation and permit observation of the effects of the added reagents. ○, ADH plus α-crystallin standard; □, plus 10 mM pantethine; ▵, plus 20 mM pantethine; •, plus 10 mM glutathione; ▴, plus 20 mM glutathione. In the presence of α-crystallin, glutathione had a stronger effect on aggregation of ADH than pantethine. (B) Effect of pantethine and glutathione on ADH only. ○, ADH only; □, plus 10 mM pantethine; ▵, plus 20 mM pantethine; •, plus 10 mM glutathione; ▴, plus 20 mM glutathione. In the absence of α-crystallin, glutathione had a stronger effect on aggregation of ADH than pantethine. All solutions were in 150 mM PBS and 2 mM EDTA (pH 7.0), at a final volume of 0.4 ml. The OD was normalized as described.