Table 2. Binding affinities (K_d, nM) of WT and redesigned CaM to selected targets.

	smMLCK	skMLCK	Spectrin	Melittin	Peptide1	CaMKI	CaMKII	CaMKK
CaMwt	1.8 ± 1.3	3.3 ± 0.8	3.3 ± 1.5	28 ± 5	1.7 ± 0.8	1.7 ± 0.7	5.1 ± 1.5	1.0 ± 3.0
CaMcore	1.3 ± 0.9	4.9 ± 1.2	16 ± 6.0	54 ± 18	147 ± 48	1.3 ± 0.8	54 ± 20	32 ± 13
Decrease in binding*	0.72	1.5	4.8	1.9	86	0.76	11	32
Specificity increase†	1.0	2.1	6.7	2.6	120	1.1	15	44
CaMmodboundarybias	2.3 ± 0.7	21 ± 3	11 ± 2	33 ± 6	343 ± 66	7 ± 2	27 ± 6	67 ± 28
Decrease in binding*	1.3	6.4	3.3	1.2	202	4.1	5.3	67
Specificity increase†	1.0	4.9	2.6	0.9	155	3.2	4.1	51

^*Decrease in binding was calculated by taking the ratio of binding affinities of a CaM variant and CaM_wt for a particular target

^†Specificity increase was calculated by taking the ratio of binding affinity of a CaM variant and CaM_wt for a particular target and dividing it by the same ratio for smMLCK