Figure 2.
Ft LVS redirects macrophage differentiation from CA-Mø to AA-Mø over time. Peritoneal macrophages from C57BL/6 mice were treated with medium only, IFN-γ (20 ng/ml) plus LPS (10 ng/ml), rIL-4 (40 ng/ml), or infected with Ft LVS (MOI = 5) for either (A) 24 or (B) 48 h. Cells were harvested and simultaneously stained for FIZZ1 and IL-12 p70. Protein expression was determined by FACS analysis. The numbers in the quadrants indicate the percent of cells within that quadrant and have been rounded to the nearest one-tenth of a percent. All treatments were performed in triplicate and data shown are from a single representative experiment (n = 3).