Table 1.

Dissociation constants between Cks1 and the different subunits.

Protein or complex	Binding partner	Kd (μM)
Cks1	p27	70
Cks1-Skp2	p27	0.47
Cks1-Cdk2	p27	120
Cks1-Cdk2-Skp2	p27	0.15
Cks1	Skp2	0.014
Cks1-Cdk2	Skp2	0.07
Cks1-p27	Skp2	0.0001
Cks1-Cdk2-p27	Skp2	0.0001
Cks1	Cdk2	1.5
Cks1-Skp2	Cdk2	8
Cks1-p27	Cdk2	3
Cks1-Skp2-p27	Cdk2	2.6

Dissociation constants in bold were derived experimentally. The remaining values were obtained through the thermodynamic cycles [19].