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p53 C-terminal peptide (DD1) is overexpressed in SH subclones. An immunoblot of total cell lysates (50 μg of total protein per lane) was probed with PAb 421 (0.1 μg/ml) (A) or PAb 1801 (0.07 μg/ml) (B). PAb 1801 does not cross-react with DD1 peptide. Four of the five stable DD1 lines are shown here.
