Table 1. Activity of peptides.
|
Kd, μM
|
Induction of
|
|||||
|---|---|---|---|---|---|---|
| Peptide | I = 0.15 M | I = 0.021 M | PAb1620* epitope induction | p53 | p21 | Apoptosis |
| FI-CDB3 | 100 | 0.6 | +++ | ++ | ++ | + |
| FI-E491/3/5/7A | 1,800 | 136 | ++ | + | + | +/- |
| FI-R490A | 140 | 0.2 | ++ | + | + | +/- |
| FI-polyGlu | 1800 | 0.01 | +++ | + | - | - |
| FI-control | NB | NB | - | - | - | - |
| Biotin-CDB3 | 800 | 12 | ND | + | + | ND |
Peptide sequences are as follows: FI-CDB3, FI-REDEDEIEW; FI-E491/3/5/7A, FI-RADADAIAW; R490A, FI-AEDEDEIEW; FI-polyGlu, FI-(Glu)9; FI-control, FI-RKSKKKITW; Biotin-CDB3, biotin-REDEDEIEW. NB, no binding observed; ND, not done.
*
Binds to the native conformation of p53