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. Author manuscript; available in PMC: 2010 Mar 30.
Published in final edited form as: J Neurosci Methods. 2008 Dec 11;178(1):128–133. doi: 10.1016/j.jneumeth.2008.12.004

Figure 2.

Figure 2

Effect of culturing rat hypothalamic slices in the absence (control) and presence of either 10ng/ml CNTF or 10ng/ml LIF in the culture medium for 14 days, on the total number of immunohistochemically identified Oxt and Avp neurons in the organotypic cultures (see Table 1 for the numbers of neurons in the individual nuclei under these conditions). Data are expressed as means ± SEM, with n=20 for controls and 18 each for CNTF- and LIF-treated cultures. Statistical differences, shown over columns by asterisks represent p values < 0.01 (compared to control), were calculated by ANOVA, followed by Fischer’s protected LSD test.