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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Arthritis Rheum. 2009 Feb;60(2):492–500. doi: 10.1002/art.24225

Figure 6. Overexpression of TRB3 decreases IGF-1 stimulation of PG synthesis.

Figure 6

Normal human chondrocytes were transfected with 5μg pcDNA 3.0 empty vector or 5μg TRB3 overexpression plasmids. The cells were allowed to recover from the transfection in monolayer culture and then placed in media containing 5% serum. 100ng/mL IGF-1 was added to indicated wells for an overnight treatment and then [35S]sulfate incorporation was performed. The counts per minute were normalized to DNA concentration of the samples and the results (mean±SEM) expressed as a % of the unstimulated control (n=3 experiments with cells from different donors). *p=0.02 control vs control+IGF-1; **p=0.02 control+IGF-1 vs TRB3+IGF-1.