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Stack plots of FTIR spectra of the amide I spectral region of the control and Smoke treated SP-B (A) and SP-C (B). Proteins (100 μg protein) were spread as a self-film in hexafluoroisopropanol(HFIP) and allowed to air dry. The film was then overlaid with HFIP solvent and spectra accumulated and processed as described in the text.
