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. 2003 Oct 29;100(23):13314–13319. doi: 10.1073/pnas.2234055100

Fig. 6.

Fig. 6.

Inhibiting Arf1 inactivation blocks the mitotic dispersion of a wide variety of peripheral Golgi proteins. (A) Interphase cells treated without or with BFA (5 μg/ml, 30 min) and mitotic cells in metaphase treated with or without H89 (50 μM) were fixed and stained with antibodies against tankyrase 1 (3T3-L1), Sak1 (HeLa), Cullin-2 (EA.hy926), myosin IIA (NRK), ankyrin 195 (NRK), and εCOPI (NRK). (Bar, 5 μm.) (B) Table summarizing the effect of short and long BFA treatments on Golgi proteins. (C) Peripheral Golgi proteins start to disperse in prophase before Golgi membranes fragment. Interphase and prophase cells (staged with Hoechst 33342) were labeled for Golgi peripheral (myosin IIA, tankyrase, and COP1) and integral membrane (GalT) proteins. ROIs were drawn around the Golgi and the whole cell. The Golgi fluorescence for each protein was background subtracted, expressed as a fraction of the total cell fluorescence, and normalized to the interphase values for 10 cells.