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. 2009 Feb 18;4(2):e4509. doi: 10.1371/journal.pone.0004509

Figure 3. Selection of E.coli host strains and media for inducible expression of hCRs.

Figure 3

A) Screening of E.coli strains and media for pEXP-hCRs. Each of pEXP-hCRs was transformed into all 5 E.coli strains: BL21, C41, C43, C41-pLysS and C43-pLysS, and spread on LB-agar plates (I), LB-agar-IPTG plates (II), TB-agar-IPTG plates (III) and 2YT-agar-IPTG plates (IV) for colony analysis. B) L-arabinose induction pBAD-hCRs for E.coli strain Top10. LB-agar plates without (−) and with (+) L-arabinose (0.05%) were used. C) Growth conditions of pBAD-hCRs in Top10 in the presence of inducer. Cell culture density at 600 nm (OD600) after indicated induction time, 3.5 hours, 6.5 hours, 10.5 hours and 24 hours, was measured.