Figure 5. Systematic detergent screens.

96 detergents were systematically screened for their ability to solubilize the Trx-hCRs protein preparation. 96 detergents were selected from the commercial Solution Master Detergent Kit and from literature. Detergents generally are divided into 4 groups depending on their chemical properties, namely, anionic (A), cationic (C), non-ionic (N) and zwitter-ionic (Z). For the majority of detergents, 1% was thus chosen as a standard concentration during the extraction trials, except for detergents with higher CMC, where ∼2% was used. As can be seen from the figure, several clusters of detergents are most effective, particularly phosphocholine (FC) series.