| pMevT |
Expresses the atoB, HMGS (S. cerevisiae ERG13), and tHMGR (S. cerevisiae HMG1) genes under control of PLAC. pACYC184 origin, Cmr; produces mevalonate from acetyl-coenzyme A |
[9]
|
| pAM25 |
As pMevT, but atoB, HMGS, and tHMGR were codon-optimized for E. coli expression and expressed under control of PLAC(UV5)
|
This work |
| pAM34 |
As pAM25, but expresses E. faecalis mvaS and mvaE
|
This work |
| pAM41 |
As pAM25, but tHMGR replaced by S. aureus mvaA
|
This work |
| pAM52 |
As pAM41, but HMGS replaced with S. aureus mvaS
|
This work |
| pMBIS |
Expresses MK, PMK, MPD, idi, and ispA under control of PLAC. pBBR1 replicon, Tcr; produces farnesyl pyrophosphate from mevalonate. |
[9]
|
| pADS |
Expresses A. annua amorphadiene synthase under control of PLAC
|
[9]
|
