Figure 5. PI3K activity is required for upregulation of early activation markers by endogenous Tim-1 on primary T cells.

Purified CD4+ T cells from C57 Bl/6 spleen and lymph node were stimulated for 24 hours with the indicated concentrations of anti-CD3 antibody (and a fixed concentration of anti-CD28 antibody - 0.5 μg/ml), with or without anti-Tim-1 antibody (6 μg/ml), in the presence or absence of PI3K inhibitor LY294002 (10 μM). Cells were stained with fluorescently labeled antibodies to CD69 (A) or CD25 (B) and analyzed by flow cytometry. Results in each panel are representative of three experiments that were performed.