. Author manuscript; available in PMC: 2009 Feb 10.

Published in final edited form as: Development. 2004 Oct 20;131(22):5599–5612. doi: 10.1242/dev.01429

Table 2.

A minority of myofibroblast progenitors from the sciatic nerve is neural crest derived

Mating	Genotype of pups	X-gal+ neural colonies	X-gal+ myofibroblast colonies
Wnt1-Cre×loxpRosa	Littermate controls	0±0%	0±0%
	Cre+, reporter+	98±6%	12±10%
Wnt1-Cre×β-actin-flox/stop-β-gal	Littermate controls	0±0%	0±0%
	Cre+, reporter+	79±18%	10±20%
P₀-Cre×β-actin-flox/stop-β-gal	Littermate controls	0±0%	0±0%
	Cre+, reporter+	52±33%	2±2%
CMV-Cre×β-actin-flox/stop-β-gal	Littermate controls	0±0%	0±0%
	Cre+, reporter+	83±24%	88±10%

Sciatic nerves were dissected from individual E13.5 pups, dissociated and cultured at clonal density under standard conditions (Morrison et al., 1999). After 5–6 days, the cultures were stained with X-gal, and the percentages of β-gal-expressing neural or myofibroblast colonies were counted. Because the CMV promoter is widely expressed in the early embryo, it serves as a positive control for reporter activation, demonstrating that the great majority of neural and myofibroblast progenitors were capable of expressing β-gal. Statistics represent mean±s.d. for at least three pups per treatment.