Fig. 3.

Persistence of pHSVth DMA and expression of human TH RNA. (A) DMA was extracted from sections and subjected to PCR with the use of primers specific to the human TH gene, and the products were electrophoresed (23). Age is the time after gene transfer a rat was analyzed (6 months, rat pHSVth no. 27; 16 months, rats pHSVth no. 2, and no. 9). Brain areas: R, right injected striatum; L, left uninjected striatum; Cb, cerebellum. Minus sign indicates no DMA; plus sign indicates pHSVth DMA isolated from Escherichia coli, which should direct production of a 186-bp fragment (number of base pairs is shown at left). (B) RT-PCR analysis of RNA isolated from specific brain areas 1 month after injection of pHSVth (14) into the right striatum. Brain areas: St, striatum; Ct, cortex; SN, midbrain (substantia nigra); Cb, cerebellum. Minus sign indicates no RNA; plus sign indicates pHSVth DNA isolated from E. coli; the methods used (24) should generate a 160-bp fragment (number of base pairs is shown at left).