Table 1.
Summary of rotation rates and histological analysis of rats receiving pHSVth, pHSVlac, or PBS.
| Histological analysis
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Decrease in baseline rotation rate* (%)
|
TH-IR§
|
X-Gal
|
TH PCR|| |
|||||||||
| Rat | Injection | Average | Final | Time of autopsy† | Microdialysis‡ | S | C | GP | S | RS | LS | CB |
| 2 | pHSVth | 57 | 56 | 16 | N | + | + + | + | + | − | − | |
| 3 | pHSVth | 93 | 97 | 12 | N | + | − | + | ND | |||
| 4 | pHSVth | 46 | 29 | 11 | N | + | + | − | + | + | ND | |
| 9 | pHSVth | 62 | 62 | 15.5 | N | + | − | − | + | − | − | |
| 26 | pHSVth | 39 | 56 | 5.5 | Y | PP¶ | + | − | ND | |||
| 27 | pHSVth | 34 | 26 | 5.5 | Y | + + | + + | + + | + | − | ND | |
| 30 | pHSVth | 26 | 16 | 6 | N | − | + + | − | ND | |||
| 31 | pHSVth | 41 | 46 | 8 | Y | + + | + | + | − | − | ND | |
| 33 | pHSVth | 66 | 70 | 6 | Y | + | − | − | + | − | ND | |
| 1 | pHSVlac | −7 | −31 | 12 | Y | + | ||||||
| 6 | pHSVlac | −11 | 0 | 12 | N | − | − | − | + + | |||
| 8 | pHSVlac | −2 | −14 | 10 | Y | ND | ||||||
| 20 | pHSVlac | 11 | 21 | 13 | Y | − | − | − | + | − | − | ND |
| 32 | pHSVlac | −10 | 13 | 11 | Y | ND | ||||||
| 12 | PBS | 14 | 20 | 13 | Y | − | − | − | − | |||
| 14 | PBS | 8 | 0 | 6 | N | |||||||
| 17 | PBS | 29 | 32 | 12 | Y | − | − | − | − | |||
| 19 | PBS | −13 | 0 | 12 | Y | − | − | − | − | |||
| 25 | PBS | −7 | −9 | 8 | Y | PP/ND | ||||||
| 38 | PBS | −5 | 3 | 12 | Y | − | − | − | − | |||
The two tests from each month of the first 3 months were averaged; those three values and the other monthly values (14) were used to calculate the average.
Months after gene transfer.
Microdialysis was done (Y, yes; N, no).
−, 0; +, 1 to 3; or + +, 4 to 20 cells contained TH immunoreactivity (TH-IR) in one or more sections (18). S, striatum; C, cortex; GP, globus pallidus; PP, poor perfusion; ND or no entry, not done.
pHSVth DNA was detected with PCR (23); RS, injected right striatum; LS, uninjected left striatum; Cb, cerebellum.
Presumably because of poor tissue preservation, attempts to detect cells with TH immunoreactivity were unsuccessful, although pHSVth DNA was detected with PCR.