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. Author manuscript; available in PMC: 2009 Feb 10.
Published in final edited form as: Gene Ther. 2002 Sep;9(18):1199–1206. doi: 10.1038/sj.gt.3301710

Figure 6.

Figure 6

hRCAAV helper cell line can mimic wt AAV gene expression. Helper plasmid pAd/AAV was transfected into 293 cells. The transfected 293 cells, untransfected i26 or D5 cells were then infected with adenovirus at the MOI of 10. Cells were harvested at the indicated time points after Ad infection. Total RNA were isolated and subjected to quantitative RTPCR using primers specific to rep and cap transcript, respectively. The figure shows the amount of rep and cap transcripts at various time points relative to its peak level.