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. 1996 Dec 24;93(26):15203–15208. doi: 10.1073/pnas.93.26.15203

Figure 2.

Figure 2

GAIP is present in both membrane and soluble fractions. A postnuclear supernatant (PN) from AtT-20 cells stably expressing GAIP (clone 14) was centrifuged at 100,000 × g to yield a crude membrane fraction (P) and a soluble fraction (S). These fractions were immunoblotted with 12CA5 mAb (anti-HA) and detected by ECL, and the amount found in the membrane and cytosolic fractions was quantified by densitometry. Most of the GAIP (80–90%) is associated with the membrane pellet, but the remainder is found in the soluble fraction.