Table 1.
GAIP interacts specifically with Gαi subfamily members
| Bait | β-gal
|
|
|---|---|---|
| Filter | Liquid, % | |
| Gαi1 | +++ | 55 |
| Gαi2 | + | 6.3 |
| Gαi3 | +++ | 100 |
| Gα0 | +++ | 52 |
| Gαz | + | 5.0 |
| Gαs | — | <1 |
| Gαs(Q224L) | — | <1 |
| Gαq | — | <1 |
| Gα13 | — | <1 |
The β-gal filter assay was performed on (Leu−, Trp−) plates, and intensity of color was scored after 8 hr. —, no color; +, weak color; +++, strong color. For the β-gal liquid assay (Leu−, Trp−), the Gαi3/GAIP interaction [12.5 Miller units (23)] was taken as 100%. Yeast cotransformed with void bait and prey vectors were taken as background. Baits were constructed in pGBT9, and GAIP prey vector was pACT2-rGAIP. For each experiment three colonies were picked. Values represent the mean of three independent experiments.