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. 2003 Oct 30;100(23):13338–13343. doi: 10.1073/pnas.2234416100

Fig. 1.

Fig. 1.

20E responsiveness in the fat body of previtellogenic female mosquitoes. (A) Hormonal titers during the first vitellogenic cycle of the anautogenous mosquito, A. aegypti. BM, blood meal; E, eclosion; JH III titers [modified from Shapiro et al. (18)]; 20E titers [modified from Hagedorn et al. (6)]. (B) Fat bodies from mosquitoes were isolated at 12-h intervals after eclosion and cultured in vitro for 6 h in medium with or without the presence of 1 × 10-6 M 20E. The expression of the genes involved in the 20E signal pathway was determined by real-time PCR and was normalized to β-actin expression. Representative data (mean ± SEM) from at least three independent experiments are shown. CM, culture medium.