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. 2003 Oct 30;100(23):13338–13343. doi: 10.1073/pnas.2234416100

Fig. 3.

Fig. 3.

Mosquito βFTZ-F1 protein in the fat body during vitellogenesis. (A) Fat-body nuclear extracts were separated on SDS/10% polyacrylamide gel, transferred to polyvinylidene fluoride membrane, and analyzed with rabbit polyclonal anti-AaβFTZ-F1 antiserum. The antibodies bound were then stripped, and the membrane was reprobed with monoclonal antibody against β-actin. TNT, in vitro synthesized AaβFTZ-F1. (B) βFTZ-F1 mRNA in the fat body of adult females was measured by real-time PCR. Representative data (mean ± SEM) from at least three independent experiments are shown. (C) βFTZ-F1 in the fat-body nuclear extracts was examined with electrophoretic gel mobility shift assay as described by Li et al. (22). The arrowhead indicates the supershifted band. F1RE, consensus binding site of βFTZ-F1; FBNE, fat-body nuclear extracts.