Fig. 4.

JH III stimulates synthesis of βFTZ-F1 in fat bodies cultured in vitro. Fat bodies of newly emerged female adults (6 h PE) were cultured in medium for 18 h in the presence of JH III or acetone. Western blot analyses were performed on fat-body nuclear extracts by using antibodies against AaβFTZ-F1 and β-actin. Fat bodies taken directly from female mosquitoes at 6 h PE and 72 h PE were used as controls. Representative data from three independent experiments are shown. CM, culture medium.