Fig. 1.

Gene structure of Elovl5 and generation of Elovl5^−/− mice. A: Gene structure of the Elovl5 wild-type and disrupted allele. Elovl5 contains 8 exons and 7 introns. The first ATG and the stop (TGA) codon reside on exon 2 and exon 8, respectively. Coding regions are shown by gray boxes. The insertion site of the gene trap vector and the splice acceptor in the vector are indicated with arrows. BglII restriction sites and the expected DNA fragments produced by BglII digestion are shown. The location of the probe for Southern blotting with BglII digestion is shown by a filled box. B: Southern blot result of BglII digested genomic DNA from wild-type (^+/+), heterozygous (^+/−), and Elovl5^−/− (^−/−) mice. C: Total RNA prepared from livers of wild-type (WT) (^+/+) and Elovl5^−/− (^−/−) mice were subjected to Northern blot analysis for elongation of very long chain fatty acids (ELOVL)5 and cyclophilin as described under Experimental Procedures. KO, knockout.