Fig. 3.

Immunoblot analysis of sterol regulatory element-binding proteins (SREBPs), SCAP, Insig-1, and Insig-2 in livers of wild-type and Elovl5^−/− mice. Membrane and nuclear proteins were prepared from livers of five wild-type and Elovl5^−/− female mice and equal aliquots from each were pooled (total, 30 μg) and subjected to SDS-PAGE. A, Immunoblotting of SREBP-1, SREBP-2, transferrin receptor (TFR), and cAMP response element binding protein (CREB) was performed using anti-mouse SREBP-1, SREBP-2, anti-human CREB, and TFR antibodies as described in Experimental Procedures. B: Immunoblotting of SREBP cleavage-activating protein (SCAP), Insig-1, Insig-2, and TFR was performed using anti-hamster SCAP, anti-mouse Insig-1, Insig-2, and anti-human TFR antibodies. Similar results were obtained in five independent experiments. ChREBP, carbohydrate regulatory element-binding protein.