TABLE 2.
Fatty acid composition of major lipid classes in livers of wild-type and Elovl5−/− mice
| Fatty Acid Compositions |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Lipid class | Genotype | C16:0 | C16:1 | C18:0 | C18:1, n-9 | C18:2, n-6 | C18:3, n-6 | C20:4, n-6 | C22:6, n-3 |
| Total lipids (μg/mg) | +/+ | 10 ± 0.2 | 0.6 ± 0.1 | 6.1 ± 0.3 | 9.5 ± 0.6 | 5.7 ± 0.3 | 0.05 ± 0.1 | 6.4 ± 0.2 | 1.9 ± 0.1 |
| −/− | 15 ± 1.1a | 2.7 ± 0.5a | 5.5 ± 0.4 | 23 ± 2.8a | 7.6 ± 0.5 | 1.2 ± 0.1a | 4.1 ± 0.1a | 1.3 ± 0.0a | |
| Total lipids (% of total) | +/+ | 24 ± 0.5 | 1.4 ± 0.2 | 15 ± 0.9 | 23 ± 1.5 | 14 ± 0.6 | 0.1 ± 0.1 | 16 ± 0.8 | 4.7 ± 0.3 |
| −/− | 24 ± 0.4 | 4.3 ± 0.6a | 9.2 ± 1.0a | 36 ± 1.6a | 13 ± 0.3 | 2.0 ± 0.1a | 6.9 ± 0.6a | 2.1 ± 0.1a | |
| Phospho-lipids (%) | +/+ | 26 ± 0.3 | 0.5 ± 0.2 | 22 ± 0.7 | 11 ± 0.4 | 13 ± 0.3 | ND | 20 ± 0.3 | 5.4 ± 0.2 |
| −/− | 26 ± 0.2 | 1.8 ± 0.2a | 18.± 0.7a | 16 ± 0.4a | 17 ± 0.2a | 2.6 ± 0.1a | 13 ± 0.6a | 3.7 ± 0.2a | |
| Triglycerides (%) | +/+ | 26 ± 0.6 | 3.1 ± 0.4 | 2.0 ± 0.1 | 45 ± 0.4 | 17 ± 1.3 | 0.5 ± 0.0 | 1.9 ± 0.1 | 0.5 ± 0.1 |
| −/− | 23 ± 0.6a | 7.4 ± 1.0a | 1.4 ± 0.1a | 50 ± 1.1a | 12 ± 0.5a | 3.1 ± 0.3a | 0.1 ± 0.1a | ND | |
| Cholesteryl esters (%) | +/+ | 33 ± 0.9 | 2.5 ± 0.3 | 4.1 ± 0.5 | 47 ± 1.0 | 7.9 ± 0.6 | ND | 1.7 ± 0.2 | ND |
| −/− | 27 ± 0.7a | 5.5 ± 0.6a | 2.5 ± 0.2a | 55 ± 0.9a | 6.3 ± 0.4 | 0.6 ± 0.1 | 0.2 ± 0.0a | ND | |
ND, not detected. Livers from 8- to 9-wk-old female mice fed a chow diet were extracted and the major classes of lipids were separated on a silica column. Lipid fractions were methyl esterified and quantified by gas-liquid chromatography (GLC). Each value represents the mean ± SE from five mice.
a
Levels of statistical significance of P < 0.05 (Student's t-test).