TABLE 3.
Expression of mRNAs in livers of Elovl5−/− mice
| mRNA | Relative Expression | |
|---|---|---|
| Elovl5 genotype | +/+ | −/− |
| SREBP pathway | ||
| SREBP-1c | 1.0 ± 0.3 | 1.1 ± 0.1 |
| SREBP-1a | 1.0 ± 0.1 | 1.1 ± 0.1 |
| SREBP-2 | 1.0 ± 0.1 | 1.2 ± 0.2 |
| Insig-1 | 1.0 ± 0.2 | 1.6 ± 0.1 |
| Insig-2 | 1.0 ± 0.1 | 2.0 ± 0.1a |
| Fatty acid synthesis | ||
| ACC-1 | 1.0 ± 0.0 | 1.9 ± 0.2a |
| FAS | 1.0 ± 0.2 | 3.0 ± 0.5a |
| ELOVL6 | 1.0 ± 0.1 | 2.3 ± 0.5 |
| SCD-1 | 1.0 ± 0.3 | 5.5 ± 0.9a |
| NADPH production | ||
| G6PD | 1.0 ± 0.3 | 5.9 ± 1.1a |
| Malic enzyme | 1.0 ± 0.2 | 3.9 ± 0.7a |
| TG synthesis | ||
| GPAT | 1.0 ± 0.2 | 3.1 ± 0.4a |
| AGPAT1 | 1.0 ± 0.1 | 0.9 ± 0.1 |
| AGPAT2 | 1.0 ± 0.1 | 1.4 ± 0.1 |
| MGAT1 | 1.0 ± 0.5 | 2.1 ± 0.5 |
| MGAT2 | 1.0 ± 0.1 | 1.4 ± 0.3 |
| DGAT1 | 1.0 ± 0.1 | 1.5 ± 0.3 |
| DGAT2 | 1.0 ± 0.2 | 1.4 ± 0.0 |
| PUFA synthesis | ||
| ELOVL1 | 1.0 ± 0.1 | 1.0 ± 0.0 |
| ELOVL2 | 1.0 ± 0.1 | 2.1 ± 0.1a |
| ELOVL3 | 1.0 ± 0.3 | 1.7 ± 0.3 |
| Δ6 desaturase | 1.0 ± 0.1 | 2.2 ± 0.0a |
| Δ5 desaturase | 1.0 ± 0.1 | 1.9 ± 0.1a |
| LXRα target genes | ||
| ABCG5 | 1.0 ± 0.2 | 1.5 ± 0.2 |
| ABCG8 | 1.0 ± 0.2 | 1.8 ± 0.2a |
| PLTP | 1.0 ± 0.2 | 5.3 ± 1.0a |
| Lipoprotein lipase | 1.0 ± 0.3 | 0.7 ± 0.1 |
| PPAR-α target genes | ||
| Acyl-CoA oxidase | 1.0 ± 0.2 | 1.4 ± 0.1 |
| Cyp4a10 | 1.0 ± 0.3 | 1.3 ± 0.1 |
| L-PBE | 1.0 ± 0.1 | 1.9 ± 0.2a |
| CD-36 | 1.0 ± 0.1 | 1.2 ± 0.1 |
| LCAD | 1.0 ± 0.1 | 1.5 ± 0.1a |
| CPT-1 | 1.0 ± 0.2 | 1.3 ± 0.1 |
| ChREBP | 1.0 ± 0.1 | 1.4 ± 0.1a |
ACC, acetyl-CoA carboxylase; AGPAT, 1-acylglycerol-3-phosphate-O-acyltransferase; ChREBP, carbohydrate regulatory element-binding protein; DGAT, diacylglycerol acyltransferase; ELOVL, elongation of very long chain fatty acids; FAS, fatty acid synthase; G6PD, glucose-6-phosphate dehydrogenase; GPAT, glycerol-3-phosphate acyl transferase; MGAT, monoacylglycerol acyltransferase; SREPB, sterol regulatory element-binding protein; SCD-1, stearoyl-CoA desaturase 1. Four female wild-type and four Elovl5−/− mice were used for the experiment. Total RNA from individual livers were subjected to quantitative real-time PCR as described in Experimental Procedures. Cyclophilin was used as the invariant control. Values represent the mean ± SE amount of mRNA from four mice relative to that of wild-type mice fed chow, which is defined as 1.
Levels statistically different than wild-type mice fed chow (P < 0.05, Student's t-test). Similar results were obtained from three independent experiments.