TABLE 4.
Fatty acid composition of livers from wild-type and Elovl5−/− mice fed diets supplemented with arachidonic acid and docosahexaenoic acid (DHA)
| Fatty Acid Concentration (μg/mg liver) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Diet | Genotype | C16:0 | C16:1 | C18:0 | C18:1, n-9 | C18:2, n-6 | C18:3, n-6 | C20:4, n-6 | C22:6, n-3 |
| Chow (4% fat) | +/+ | 11.4 ± 0.9 | 0.8 ± 0.1 | 5.7 ± 0.3 | 13.8 ± 1.5 | 6.3 ± 0.4 | ND | 6.1 ± 0.2 | 2.5 ± 0.1 |
| −/− | 15.8 ± 0.9a | 3.3 ± 0.5a | 4.9 ± 0.2 | 27.8 ± 2.6a | 8.0 ± 0.4a | 1.5 ± 0.1a | 4.1 ± 0.1a | 1.9 ± 0.1a | |
| 0.5% Arachidonic acid | +/+ | 8.4 ± 0.2a | 0.3 ± 0.0a | 5.7 ± 0.3 | 6.6 ± 0.5a | 4.6 ± 0.2a | ND | 8.9 ± 0.2a | 2.4 ± 0.1 |
| −/− | 11.2 ± 0.5 | 1.0 ± 0.1 | 5.6 ± 0.1 | 13.1 ± 1.7 | 5.0 ± 0.6 | 0.6 ± 0.1a | 9.5 ± 0.3a | 1.2 ± 0.1a | |
| 0.25% DHA | +/+ | 9.0 ± 0.5 | 0.2 ± 0.1a | 6.2 ± 0.2 | 6.2 ± 0.7a | 6.0 ± 0.3 | ND | 4.7 ± 0.1a | 7.2 ± 0.3a |
| −/− | 12.7 ± 1.2 | 1.1 ± 0.2 | 5.6 ± 0.3 | 14.0 ± 2.0 | 8.0 ± 0.6a | 0.6 ± 0.1a | 3.0 ± 0.2a | 7.7 ± 0.4a | |
| 0.2% Arachidonic acid + 0.05% DHA | +/+ | 8.3 ± 0.4a | 0.4 ± 0.0a | 5.4 ± 0.3 | 6.8 ± 0.5a | 5.0 ± 0.2 | ND | 6.9 ± 0.2a | 3.3 ± 0.2a |
| −/− | 12.4 ± 0.7 | 1.3 ± 0.1a | 5.6 ± 0.4 | 14.2 ± 0.8 | 6.0 ± 0.3 | 0.7 ± 0.1a | 6.9 ± 0.3a | 3.2 ± 0.1a | |
Livers from 11- to 15-week-old female mice fed the indicated diets for 7 days were extracted. Fatty acids were methyl esterified and quantified by GLC. Each value represents the mean ± SE from five mice.
a
Levels statistically different than wild-type mice fed chow (P < 0.05, Student's t-test).