TABLE 5.
Relative amounts of mRNAs in livers from wild-type and Elovl5−/− mice on diets supplemented with unsaturated fatty acids
| Diets | Chow | 0.2% Arachidonic + 0.05% DHA | ||
|---|---|---|---|---|
| Elovl5 genotype | +/+ | −/− | +/+ | −/− |
| SREBP pathway | ||||
| SREBP-1c | 1.0 ± 0.2 | 1.3 ± 0.2 | 0.7 ± 0.1 | 1.1 ± 0.2 |
| SREBP-1a | 1.0 ± 0.1 | 1.3 ± 0.1 | 1.0 ± 0.1 | 1.1 ± 0.0 |
| SREBP-2 | 1.0 ± 0.2 | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.8 ± 0.1 |
| Insig-1 | 1.0 ± 0.1 | 1.3 ± 0.1 | 0.7 ± 0.1 | 0.7 ± 0.1 |
| Insig-2 | 1.0 ± 0.1 | 1.7 ± 0.1a | 0.9 ± 0.0 | 1.0 ± 0.0 |
| Fatty acid synthesis | ||||
| ACC-1 | 1.0 ± 0.2 | 2.3 ± 0.4a | 0.8 ± 0.1 | 1.1 ± 0.1 |
| FAS | 1.0 ± 0.3 | 2.8 ± 0.4a | 0.5 ± 0.1 | 0.9 ± 0.2 |
| SCD-1 | 1.0 ± 0.2 | 4.6 ± 0.8a | 0.6 ± 0.2 | 1.1 ± 0.1 |
| G6PD | 1.0 ± 0.2 | 7.8 ± 1.4a | 0.7 ± 0.1 | 1.3 ± 0.2 |
| Malic enzyme | 1.0 ± 0.2 | 2.7 ± 0.3a | 0.5 ± 0.1 | 1.4 ± 0.2 |
| ELOVL2 | 1.0 ± 0.1 | 1.6 ± 0.1a | 0.6 ± 0.0a | 0.8 ± 0.1 |
| ELOVL6 | 1.0 ± 0.2 | 2.8 ± 0.2a | 0.5 ± 0.1 | 1.0 ± 0.2 |
| Δ6 desaturase | 1.0 ± 0.1 | 2.4 ± 0.3a | 0.8 ± 0.1 | 0.9 ± 0.1 |
| Δ5 desaturase | 1.0 ± 0.1 | 1.8 ± 0.2a | 0.8 ± 0.1 | 0.9 ± 0.1 |
Total RNA from each liver of five mice used in Table 4 was subjected to quantitative real-time PCR as described in Experimental Procedures. Values represent the mean ± SE amount of mRNA from five mice relative to that of wild-type mice fed chow, which is defined as 1. Cyclophilin was used as the invariant control.
a
Levels statistically different than wild-type mice fed chow (P < 0.05, Student's t-test).