Fig. 2.

The ratio of liver weight to body weight, body weight loss, and liver function tests in wild-type and S1P₂^−/− mice in acute liver injury induced by CCl₄. A single injection of CCl₄ was performed in wild-type (WT) and S1P₂^−/− mice. The ratio of liver weight to body weight was measured in untreated wild-type and S1P₂^−/− mice from 24 to 96 h at euthanization after CCl₄ injection (A). The alteration of body weight was determined by comparison of body weights before CCl₄ injection with those at 24, 48, 72, and 96 h after CCl₄ injection (B). Serum concentrations of alanine aminotransferase (ALT) (C) and alkaline phosphatase (ALP) (D) were measured in wild-type and S1P₂^−/− mice at 24, 48, 72, and 96 h after CCl₄ injection. Columns and bars represent means ± SEM of four animals. The asterisk indicates a significant difference from wild type in Student's t-test (P < 0.05).