Table 2.

Quantitative immunofluorescence analysis of Cx45 colocalization relationships with Cx36, ZO-1, ZO-2, and ZONAB in the IPL of adult mouse retina

Proteins examined	Protein(total puncta counted)a	Percentagecolocalizationb
Cx45 positive for Cx36c	Cx45 (766)	46 ± 7.2
Cx45 positive for Cx36d	Cx45 (2952)	87 ± 1.3
Cx45 positive for ZO-1	Cx45 (737)	71 ± 2.8
Cx45 positive for ZO-2	Cx45 (432)	7.0 ± 2.1
Cx45 positive for ZONAB	Cx45 (472)	4.7 ± 0.4

^aValues in parentheses represent the total number of Cx45-positive or Cx36-positive puncta counted in 4–10 separate single-scan confocal images of the IPL in retinas from two to four mice.

^bValues represent means ± SEM.

^cData from mice prepared with 40 ml of fixative, with immediate washout.

^dData from mice prepared with 20 ml of fixative, with immediate washout.