. 2008 Sep 24;28(39):9769–9789. doi: 10.1523/JNEUROSCI.2137-08.2008

Table 3.

Numbers of gap junctions labeled for Cx36, Cx45, or Cx36+Cx45 in FRIL single-replicas of mouse and rat retinas that were double labeled for Cx36 plus Cx45

Antibody	Mouse		Rat	Total
Antibody	Shorter labeling^a	Longer labeling^b	Shorter labeling	Total
Cx36	408 (92%)	137 (88%)	62 (85%)	607 (90%)
Cx36+Cx45	29 (7%)	19 (12%)	10 (14%)	58 (9%)
Cx45	5 (1%)	0 (0%)	1 (1%)	6 (1%)
Total	442 (100%)	156 (100%)	73 (100%)	671 (100%)

Numbers in parentheses indicate percentage of labeled gap junctions found in each labeling protocol. These data do not include the 160-plus Cx36 and Cx36+Cx45-containing gap junctions found in the double replicas (see Table 4).

^aShorter labeling was defined as 1–4.4 h for primary and 12–17 h for secondary antibody labeling; no significant differences were noted between 1–1.2 h and 4.4 h for primary antibodies.

^bLonger labeling was defined as 5.3–6.8 h for primary and 21.5 h for secondary antibodies.