Table 4.

Numbers of gap junctions in single and double replicas, percentage of Cx45+Cx36 double-labeled gap junctions, and lower and upper confidence intervals at 95% confidence levels for Cx45-containing gap junctions expressing both connexins

Observed	Percentage	Lower 95% CI	Upper 95% CI
19/19a	100.00%	85.4%	100.0%
41/45b	91.1%	78.8%	97.0%
60/64c	93.8%	84.8%	98.3%
20/22d	90.9%	70.8%	98.9%
8/10e	80%	44.4%	97.5%

CI, Confidence interval.

^aCx45+Cx36-containing gap junctions from three FRIL replicas that were labeled for longer times in both primary and secondary antibodies.

^bCx45+Cx36-containing gap junctions from the other 15 FRIL replicas.

^cCx45+Cx36-containing gap junctions, data combined from all 18 FRIL replicas.

^dCx45+Cx36-containing gap junctions from 1 unpaired and 11 paired hemiplaques, calculated as individual hemiplaques, from one SDS-FRL double-replica.

^eCx45+Cx36-containing gap junctions from paired matched hemiplaques, calculated as pairs, from one SDS-FRL double-replica. (Not included is the pair with label for Cx45 and Cx36 on one side and neither label on the other side.)