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Cyclin E is the predominant cyclin in p107/E2F complexes in tumor cells. E2F complexes were analyzed by gel retardation assays using cell lysates (15 μg) prepared from synchronized populations (see Fig. 2) of normal 76N cells (A) and tumor MDA-MB-157 cells (B). The oligonucleotide used as a labeled DNA probe includes the E2F binding site of the human dehydrofolate reductase promoter. The anti-cyclin E antibody (200 ng) was used to disrupt the E2F complexes.
