Table 1.
Characterization of normal and tumor-derived breast epithelial cells
| Cell lines | Cell types | Estrogen receptor (31) | p53 | Cyclin E (31, 32) | pRb* |
|---|---|---|---|---|---|
| 1. 70N | N-mortal | − | + (39) | + | + |
| 2. 81N | N-mortal | − | + (39) | + | + |
| 3. 76N | N-mortal | − | + (39) | + | + |
| 4. MCF-10-A | N-immortalized | − | + (40) | + | + |
| 5. MCF-7 | A (pe) | + | + (40) | +++ | + |
| 6. MDA-MB-157 | C (pe) | − | − (40) | ++++++ | + |
| 7. MDA-MB-231 | A (pe) | − | − (40) | ++++ | + |
| 8. MDA-MB-436 | A | − | − (41) | +++++ | − |
| 9. T47D | DC (pe) | + | − (40) | ++ | + |
| 10. BT20 | C | + | + (40) | ++ | + |
| 11. HBL-100 | T (bm) SV40 transformed | − | − (40) | +++ | − |
| 12. HS-578T | DC | − | − (40) | ++++ | − |
| 13. ZR75T | IDC | + | + (40) | +++ | + |
Cell type, estrogen receptor (ER), p53, and cyclin E status as determined in indicated references. +, wild type; ++(++++), varying degrees of overexpression with MDA-MB-157 showing the highest degree (64-fold, hence 6 + s) of cyclin E overexpression. N, normal breast cells from reduction mammoplasty; A, adenocarcinoma; pe, pleural effusion; C, carcinoma; DC, ductal carcinoma; T(bm), tumor breast milk; SV40, simian virus 40; IDC, infiltrating DC; −, mutant or not expressed.
*
pRb status is adopted from Fig. 1, in which + indicates wild type and present in hypo- and hyperphosphorylated forms, and − indicates mutated or virally bound and inactive.