Fig. 4.

Osteogenic lineage induction by GLI2 alleles in C3H10T1/2 cells. (a) WT and mutant alleles were transfected into cells, and the encoded proteins were detected by using an antibody against the N-terminal myc tag. (b) AP activity is detected when C3H10T1/2 differentiates into an osteogenic lineage. (c) Plot of the mean ± SEM of ratios of AP-positive cells per total transfected cells in 10 independent fields of cells, with one representative experiment shown. The apparent polymorphisms behave similarly to the normal control. The mutant alleles (nos. 2, 5, 8, 4, 15 and 16) lose the ability to induce AP activity.