Fig. 3.

ChIP of pSI-binding sites. (A) Shown is a Western blot of third-instar larval proteins isolated from three different su(Hw) genotypes probed with a Su(Hw) Ab. The v/f allele represents su(Hw)^v/su(Hw)^f heterozygotes, and v/v represents su(Hw)^v/su(Hw)^v flies. N.E. corresponds to embryonic nuclear extract from CS. Sizes in kilodaltons are shown on the left. (B) Representative examples of PCR products obtained from chromatin material that was directly purified (Input, IN) or immunoprecipitated with Su(Hw) or nonspecific (N.S.) Ab. (C) The average of at least three ChIP experiments is displayed. Results are expressed as percentage of input DNA. Gray bars, Su(Hw); black bars, nonspecific Ab.