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Impaired LPS-induced TNF-α production from IFN-β-/- BMMs. BMMs from IFN-β+/+ and IFN-β-/- mice were cultured for 24 h after LPS stimulation (10 and 100 ng/ml), in the presence or absence of rIFN-β. Supernatants were assayed for TNF-α in triplicate by ELISA. Data are representative of the mean ± SEM of two independent assays. (IFN-β+/+ n = 5; IFN-β-/- n = 5). ND, not detected; ***, P ≤ 0.0001.
