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. 2003 Oct 30;100(23):13495–13500. doi: 10.1073/pnas.2334503100

Fig. 1.

Fig. 1.

Analysis of expression of markers by ES cells, in vitro selectively enriched progenitor cells, and cells after in vitro differentiation. (A) RT-PCR-based analysis of expression of marker transcripts. Enriched progenitor cells were analyzed after expansion with bFGF. Differentiated cells were analyzed after 14 days of in vitro differentiation. Expression analysis of the ubiquitously expressed GAPDH is shown for reference. (B) Virtually all selected progenitors express nestin. (C) The majority of cells display Pax2 immunoreactivity. Nuclei are visualized with blue 4,6-diamidino-2-phenylindole staining. (D) After 14 days of in vitro differentiation, nuclear immunoreactivity for the hair cell marker Math1 was detectable. (E) A subset of differentiated cells express the hair cell marker myosin VIIA. (F) The hair-bundle marker espin is detectable in differentiated cells. (G) The hair cell marker parvalbumin 3 is detectable in differentiated cells.