Fig. 1.

Cytotoxicity of Chol:MβCD. Subconfluent mouse SMCs were treated with increasing concentrations of Chol:MβCD in 0.2% BSA for 72 h. Cells were then incubated for 1 h with CellTiter 96 Aqueous One Solution Reagent, convertible to a colored formazan product by metabolically active cells. (A) Relative metabolic activity was determined by spectrophotometric measurement (OD = 490 nm) of the accumulation of the formazan product in the medium. (B) Total cellular protein was determined by using a Sigma kit. See Materials and Methods for details. Data are averages ± SEM from triplicate wells and repeated at least once.