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. 2003 Oct 27;100(23):13531–13536. doi: 10.1073/pnas.1735526100

Fig. 4.

Fig. 4.

QRT-PCR determination of SMC and macrophage-related gene expression in SMCs. Subconfluent mouse SMCs were treated with (Chol) or without (Control) Chol:MβCD (10 μg/ml) in 0.2% BSA (72 h). In C, control or cholesterol-loaded cells were treated with 30 ng/ml TNF-α for 2 h after cholesterol loading. Total RNA was extracted and subjected to QRT-PCR analysis of smooth muscle marker genes (A), macrophage marker genes (B), and macrophage-related inflammation genes (C). All data are averages ± SEM from independent duplicates.